Z scores (see Methods) for two alignments, Alignment Aof Fig.
Methods PCR Analysis of APBA2 Clones and Expression PCR assays were performed with Roche Taq DNA polymerase (Indianapolis, IN) in 50 μL reactions for 30 cycles under standard conditions and T aOf 63°C, using 2.5 mM dNTPs and 0.5 μM primers.
The reader should also be alert to the possibility of confusing "A-pocket", which is shorthand for "adenosine-binding pocket", with the term "half-site A", which refers to those amino acids involved in binding RNA on subunit Aof the coat protein dimer.
For this purpose, two probes were used; one was β-actin, the other was choA, which is clone Aof a group of highly expressed mRNAs from Chinese hamster ovary (cho) cells [ 40 ] .
Subunit Aof the vacuolar-type H +-ATPase is the catalytic subunit.
Cloning, sequencing and characteristics of subunit Aof the V-Type ATPase from Arabidopsis thaliana have been previously reported [ 24 ] .
The cDNA was identified as subunit Aof the vacuolar type H +-ATPase via identity with other previously sequenced subunit A genes from other organisms.
Genome blot analysis was utilized to determine the number of genes in the A. thaliana genome corresponding to subunit Aof the vacuolar type ATPase.
Hybridization was conducted at moderate stringency with a 300 base pair homologous A. thaliana cDNA probe corresponding to subunit Aof the vacuolar type ATPase.
Since A. thaliana seemed to possess only a single gene for subunit Aof the V-ATPase it was expected that this gene should be expressed in all organs tested.
Subunit Aof the vacuolar type H +-ATPase in A. thaliana was shown to exhibit multi site polyadenylation.
Conclusions Evidence gathered to date indicates the presence of a single gene encoding subunit Aof the vacuolar type H +-ATPase in A. thaliana.
Most flowering plants evaluated to date have a small multigene family encoding subunit Aof the vacuolar type H +-ATPase.
Multi site polyadenylation does not appear to be a mechanism for posttranscriptional control of gene expression for subunit Aof the vacuolar type H +-ATPase in A. thaliana for the stress conditions and developmental stage tested.
Proton concentration changes in the aqueous bulk phase were measured with the pH sensitive dye cresol red, which has a pK aof 8.2-8.5.
In this view, the principal role of E194 and E204 in bR may to modulate the pK aof the H +-release group in the M state to a value lower than the pH of the organism's external environment.
Instead, it now seems more likely that E204 and E194 merely help to lower the pK aof the H +release group from above 8, the apparent value for pR, into the vicinity of 6 for bR.
The only way that the H +release mechanism can be strongly conserved between bR and pR is if arginine itself serves as the principal donor group for fast H +release in both, with nearby residues (such as E194 and E204) merely modulating the pK aof the arginine in the M intermediate.
However, it remains unclear how the pK aof Arg-82 in bR could be made sufficiently low in its M intermediate to serve as the H +release group at pH values down to 6.0.
1) They should present a functional group within 5 Aof beta2 Ala135.
Further optimization with flexible ligand and full atom representation of the receptor was carried out by local energy minimization, where all side-chains within 6 Aof the ligand were flexible [ 18 ] .
lower) than -35 were pre-selected for a second automatic round of selection: only ligands within 5 Aof Ala 135 of the beta-2 subunit and that were hydrogen-bonded to the carbonyl oxygen of beta-2 Ser 133 were retained.
The LAT senses a Watergate-like trail of money here, quoting Charles Lewis of the Washington-based watchdog group, the Center for Public Integrity, saying, "Wire transfers of the kind described are Exhibit Aof criminality."
Exhibit Aof something in evidence but not in the report is Lewinsky's statement to the grand jury that "no one ever asked me to lie and I was never promised a job for my silence."
If you missed the links in the piece, click to read , aof Colored People , about the in the Dictionary of Global Culture , a short in the Dictionary , and a recap of the for original scholarship rests.
A huge blowup of aof the man in his garden towers over the entryway.
The most interesting post of the week, however, emerged in aof the Crusades that took on a little-known tribe called the Cathars and their 13 th century Inquisition.
On the other hand, the pronunciation of Afghanistan in Britain follows either the pattern of Pakistan , which has the aof man in the first syllable and that of father in the last, or, as in America, is heard with the a of man in both positions.
On the other hand, the pronunciation of Afghanistan in Britain follows either the pattern of Pakistan , which has the a of man in the first syllable and that of father in the last, or, as in America, is heard with the aof man in both positions.
To be sure, the RP practice of rhyming can with man and the aof can't with that of father does neaten things up a bit.
As the FBI agent observed in his email, Part Aof the 1995 procedures applied only to information obtained pursuant to a FISA warrant.
Three scenarios can explain the presence in species Aof a gene which is absent in species B and C: first, the gene was present in the common ancestor of the species A, B, and C, and has then been independently lost in species B and C; second, the gene has been acquired by the common ancestor of species A and B, and then lost in species B; and third, the gene has been recently acquired by species A.
Indeed, the absence in species Aof a gene present in species B and C can be interpreted as the loss of the gene in species A or two independent acquisitions in species B and C. Note that apparent gene losses in A may be overestimated if recombination occur frequently between B and C (that is, acquisition of genes by B and C is not independent), however the effect of such a recombination event is probably low in the present cases (see 'Genomes' section).
In the lipid Aof B. cepacia , one of these phosphates is modified by a phosphodiester-linked 4-amino-4-deoxyarabinose residue [ 58 59 ] .
Complaint In November 2001 the Commissioner received a complaint from Dr Aof the Accident Compensation Corporation (ACC) about health care services provided to Mr B by Dr C and a public hospital.
I listened to the description on Side Aof the Attitude Activator, and was a bit skeptical, but gave the session a try.
With continuous stocking, those cattle would starve on any 0.5 Aof pasture for a day (except for the spring flush).
Last month, in Part Aof this column, we discussed the recovery of domain names by way of High Court proceedings.
In cases where such facilities will be constructed or significantly modified, in addition to the prevention measures outlined in section Aof Part V the following reduction measures could also be considered in determining best available techniques: 209178
Natural Resources Regional Plan The Air Plan will be part Aof this document.
Progress a marine reserve application for Area Aof Option 3 with the seaward boundary extended to include Shearer Rock and Marker Buoy (as in Option 1).
Dr G did not consider Dr B was negligent in not warning Ms Aof this possibility.
Side Aof this audiotape also contains the simpler "La Chi" process which can be used by beginners.
Refer to Appendix Aof the Commission's Guide to Making an Application Under the Overseas Investment Regulations 1995 for the relevant application fee.
The HFSP hazardous substances classification system is contained in Appendix Aof the Red Book, together with applicable Hazard Ratings in each Effect Type.
The Applicant applied to register the trade mark FESTIVAL OF FLOWERS in Part Aof the register in relation to fundraising services.